Factual error: When we see that Dodrio's neck is tangled, the configuration doesn't make sense. It just looks like two bands of neck, but it doesn't reflect what it would actually look like if it was really tangled.
Continuity mistake: Jessie seems completely surprised when she's told that Voltorb can explode, but in The Ninja-Pokemon Showdown (Season 1, Episode 32), not only did she and the others find out what Voltorb are, they were all affected by the explosions as well.
Audio problem: After Dr. Proctor says that he is off-duty, his mouth moves, but no sound can be heard. (00:05:39)
Continuity mistake: When Dr. Proctor says that he has been unable to refuse requests from young girls, the yellow clips on Misty's suspenders turn red like the suspenders. (00:03:22)
Other mistake: When Pikachu's pain increases, his nose is missing. (00:01:51)
Continuity mistake: When Ash, Brock, and Misty rush to find a Pokémon Center for Pikachu because he has fainted, they leave their backpacks behind. When they arrive at the hospital, they suddenly have their backpacks with them. (00:02:26)
Continuity mistake: When Ash realises that something happened with Pikachu, his bag is below the second-highest plank on the bench, but in the next shot, it is aligned with it. (00:01:27)
Answer: Yes it ran away because Layla hurt its feelings.