Plot hole: When April O'Neil calls Vernon Fenwick (drives the channel 6 van), he answers his phone and immediately says "O'Neil". When the scene cuts back to O'Neil, we see she called from a payphone. Fenwick would have no way to know it was her calling.
Plot hole: On the night after April meets the turtles for the first time, she has her boss come to her apartment and tries desperately to try and convince her that the turtles are real, unfortunately her boss wasn't convinced and she ends up getting fired. However, April had already taken two pictures of the turtles on her phone the night previously (before meeting the turtles and after they had left the roof and began jumping over other roof tops). She could have easily just shown the pictures to her boss and she would have been convinced the turtles were real. Even if Donatello deleted the picture she had taken before meeting the turtles, April had still taken a picture of the turtles after they left the roof and jumped from other roof tops, so she still could have easily been able to prove they exist.
Plot hole: The turtles are taken away to have their blood drained for the mutagen but Splinter is left behind. How come Splinter wasn't taken? He also has the mutagen in his blood.
Suggested correction: Because April told Sacks that she saved the turtles she never said anything about Splinter being saved as well. So Sacks assumed that only the turtles survived and Splinter didn't.
Suggested correction: That isn't a plot hole. Vernon assumed April was the person calling him and was correct. People do this all the time in real life.
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